Making standards for quantitative real-time pneumococcal PCR

Making standards for quantitative real-time pneumococcal PCR

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable...

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Bibliographic Details
Main Authors:	Susan C. Morpeth, Jim F. Huggett, David R. Murdoch, J. Anthony G. Scott
Format:	Article
Language:	English
Published:	Elsevier 2014-12-01
Series:	Biomolecular Detection and Quantification
Subjects:	Quantitative Real-time PCR Streptococcus pneumoniae Standards
Online Access:	http://www.sciencedirect.com/science/article/pii/S2214753514000114

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