Making standards for quantitative real-time pneumococcal PCR
Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-12-01
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| Series: | Biomolecular Detection and Quantification |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214753514000114 |