Making standards for quantitative real-time pneumococcal PCR

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable...

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Main Authors:	Susan C. Morpeth, Jim F. Huggett, David R. Murdoch, J. Anthony G. Scott
Format:	Article
Language:	English
Published:	Elsevier 2014-12-01
Series:	Biomolecular Detection and Quantification
Subjects:	Quantitative Real-time PCR Streptococcus pneumoniae Standards
Online Access:	http://www.sciencedirect.com/science/article/pii/S2214753514000114

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spelling	doaj-c748bc4a0b234487bb956268f11fdf002020-11-25T03:27:50ZengElsevierBiomolecular Detection and Quantification2214-75352014-12-012C1310.1016/j.bdq.2014.11.003Making standards for quantitative real-time pneumococcal PCRSusan C. Morpeth0Jim F. Huggett1David R. Murdoch2J. Anthony G. Scott3KEMRI-Wellcome Trust Research Programme, Kilifi, KenyaMolecular & Cell Biology, LGC, London, United KingdomDepartment of Pathology, University of Otago, Christchurch, New ZealandKEMRI-Wellcome Trust Research Programme, Kilifi, KenyaQuantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.http://www.sciencedirect.com/science/article/pii/S2214753514000114QuantitativeReal-time PCRStreptococcus pneumoniaeStandards
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Susan C. Morpeth Jim F. Huggett David R. Murdoch J. Anthony G. Scott
spellingShingle	Susan C. Morpeth Jim F. Huggett David R. Murdoch J. Anthony G. Scott Making standards for quantitative real-time pneumococcal PCR Biomolecular Detection and Quantification Quantitative Real-time PCR Streptococcus pneumoniae Standards
author_facet	Susan C. Morpeth Jim F. Huggett David R. Murdoch J. Anthony G. Scott
author_sort	Susan C. Morpeth
title	Making standards for quantitative real-time pneumococcal PCR
title_short	Making standards for quantitative real-time pneumococcal PCR
title_full	Making standards for quantitative real-time pneumococcal PCR
title_fullStr	Making standards for quantitative real-time pneumococcal PCR
title_full_unstemmed	Making standards for quantitative real-time pneumococcal PCR
title_sort	making standards for quantitative real-time pneumococcal pcr
publisher	Elsevier
series	Biomolecular Detection and Quantification
issn	2214-7535
publishDate	2014-12-01
description	Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.
topic	Quantitative Real-time PCR Streptococcus pneumoniae Standards
url	http://www.sciencedirect.com/science/article/pii/S2214753514000114
work_keys_str_mv	AT susancmorpeth makingstandardsforquantitativerealtimepneumococcalpcr AT jimfhuggett makingstandardsforquantitativerealtimepneumococcalpcr AT davidrmurdoch makingstandardsforquantitativerealtimepneumococcalpcr AT janthonygscott makingstandardsforquantitativerealtimepneumococcalpcr
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Making standards for quantitative real-time pneumococcal PCR

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