A novel method for expansion and differentiation of mouse tracheal epithelial cells in culture
Abstract Air-liquid interface (ALI) cultures of mouse tracheal epithelial cells (MTEC) are a well-established model to study airway epithelial cells, but current methods require large numbers of animals which is unwanted in view of the 3R principle and introduces variation. Moreover, stringent breed...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2018-05-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-018-25799-6 |