A novel method for expansion and differentiation of mouse tracheal epithelial cells in culture

Abstract Air-liquid interface (ALI) cultures of mouse tracheal epithelial cells (MTEC) are a well-established model to study airway epithelial cells, but current methods require large numbers of animals which is unwanted in view of the 3R principle and introduces variation. Moreover, stringent breed...

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Bibliographic Details
Main Authors: Evelien Eenjes, Tinne C. J. Mertens, Marjon J. Buscop-van Kempen, Yolanda van Wijck, Christian Taube, Robbert J. Rottier, Pieter S. Hiemstra
Format: Article
Language:English
Published: Nature Publishing Group 2018-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-018-25799-6