Live-Cell Cardiac-Specific High-Throughput Screening Platform for Drug-Like Molecules that Enhance Ca<sup>2+ </sup>Transport

Live-Cell Cardiac-Specific High-Throughput Screening Platform for Drug-Like Molecules that Enhance Ca<sup>2+ </sup>Transport

We engineered a concatenated fluorescent biosensor and dual-wavelength fluorescence lifetime (FLT) detection, to perform high-throughput screening (HTS) in living cells for discovery of potential heart-failure drugs. Heart failure is correlated with insufficient activity of the sarcoplasmic reticulu...

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Bibliographic Details
Main Authors: Tory M. Schaaf, Evan Kleinboehl, Samantha L. Yuen, Lauren N. Roelike, Bengt Svensson, Andrew R. Thompson, Razvan L. Cornea, David D. Thomas
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Cells
Subjects:
time-resolved FRET
biosensor
drug screening
SERCA2a
small-molecule
HTS
Online Access:https://www.mdpi.com/2073-4409/9/5/1170

Internet

https://www.mdpi.com/2073-4409/9/5/1170

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