Improving the Thermostability of Acidic Pullulanase from Bacillus naganoensis by Rational Design.

Pullulanase (EC 3.2.1.41) plays an important role in the specific hydrolysis of branch points in amylopectin. Enhancing its thermostability is required for its industrial application. In this study, rational protein design was used to improve the thermostability of PulB from Bacillus naganoensis (AB...

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Bibliographic Details
Main Authors: Meihui Chang, Xiaoyu Chu, Jinzhi Lv, Qingbin Li, Jian Tian, Ningfeng Wu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5072709?pdf=render