Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing
ESRG was first identified in our previous study. It is highly expressed in human embryonic stem cells (hESCs), whereas it is significantly down-regulated in differentiated cells and is undetectable in adult tissues. To develop an hESC line for monitoring the expression of ESRG for further study of i...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-10-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120302841 |
| id |
doaj-c4fb1693dc8a4dc9a7aa8008f049596e |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Hui Liu Shasha Li Caiping Ren Weidong Liu Bin Zhu Lei Wang Hongjuan Xu Wen Xie Xiang Zuo Yao Zhou Longlong Luo Xingjun Jiang |
| spellingShingle |
Hui Liu Shasha Li Caiping Ren Weidong Liu Bin Zhu Lei Wang Hongjuan Xu Wen Xie Xiang Zuo Yao Zhou Longlong Luo Xingjun Jiang Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing Stem Cell Research |
| author_facet |
Hui Liu Shasha Li Caiping Ren Weidong Liu Bin Zhu Lei Wang Hongjuan Xu Wen Xie Xiang Zuo Yao Zhou Longlong Luo Xingjun Jiang |
| author_sort |
Hui Liu |
| title |
Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing |
| title_short |
Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing |
| title_full |
Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing |
| title_fullStr |
Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing |
| title_full_unstemmed |
Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing |
| title_sort |
generation of an esrg pr-tdtomato reporter human embryonic stem cell line, csue011-a, using crispr/cas9 editing |
| publisher |
Elsevier |
| series |
Stem Cell Research |
| issn |
1873-5061 |
| publishDate |
2020-10-01 |
| description |
ESRG was first identified in our previous study. It is highly expressed in human embryonic stem cells (hESCs), whereas it is significantly down-regulated in differentiated cells and is undetectable in adult tissues. To develop an hESC line for monitoring the expression of ESRG for further study of its function, we used gene editing techniques to insert fusion sequences of ESRG promoter and tdTomato fluorescent protein gene into the AAVS1 human safe harbor locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiation potential. |
| url |
http://www.sciencedirect.com/science/article/pii/S1873506120302841 |
| work_keys_str_mv |
AT huiliu generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT shashali generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT caipingren generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT weidongliu generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT binzhu generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT leiwang generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT hongjuanxu generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT wenxie generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT xiangzuo generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT yaozhou generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT longlongluo generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing AT xingjunjiang generationofanesrgprtdtomatoreporterhumanembryonicstemcelllinecsue011ausingcrisprcas9editing |
| _version_ |
1724530852878090240 |
| spelling |
doaj-c4fb1693dc8a4dc9a7aa8008f049596e2020-11-25T03:41:14ZengElsevierStem Cell Research1873-50612020-10-0148101983Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editingHui Liu0Shasha Li1Caiping Ren2Weidong Liu3Bin Zhu4Lei Wang5Hongjuan Xu6Wen Xie7Xiang Zuo8Yao Zhou9Longlong Luo10Xingjun Jiang11Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; Laboratory of Stem Cell & Retinal Regeneration, Institute of Stem Cell Research, Division of Ophthalmic Genetics, The Eye Hospital, Wenzhou Medical University, Wenzhou, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, ChinaCancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, ChinaCancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, China; Corresponding authors at: Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China (C. Ren); State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, China (L. Luo); Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China (X. Jiang).Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, ChinaCancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, ChinaCancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, ChinaCancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, China; Key Laboratory of Biological Nanotechnology of National Health Commission, Xiangya Hospital, Central South University, Changsha, ChinaCancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, ChinaCancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, ChinaCancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, ChinaState Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, China; Corresponding authors at: Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China (C. Ren); State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, China (L. Luo); Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China (X. Jiang).Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China; Corresponding authors at: Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China (C. Ren); State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, China (L. Luo); Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China (X. Jiang).ESRG was first identified in our previous study. It is highly expressed in human embryonic stem cells (hESCs), whereas it is significantly down-regulated in differentiated cells and is undetectable in adult tissues. To develop an hESC line for monitoring the expression of ESRG for further study of its function, we used gene editing techniques to insert fusion sequences of ESRG promoter and tdTomato fluorescent protein gene into the AAVS1 human safe harbor locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiation potential.http://www.sciencedirect.com/science/article/pii/S1873506120302841 |