The Effect of Different Matrix Bound on the Transesterification Activity of Immobilized PPD2 Lipase

• Main Authors: Anita Herawati Permana, Fida Madayanti Warganegara, Deana Wahyuningrum, Akhmaloka
• Format: Article
• Language: English
• Published: Journal of Pure and Applied Microbiology 2018-06-01
• Series: Journal of Pure and Applied Microbiology
• Subjects: geobacillus thermoleovorans ppd2; thermostable lipase; immobilization; ni-nta agarose; carboxmethyl chitosan; transesterification.
• Online Access: https://microbiologyjournal.org/the-effect-of-different-matrix-bound-on-the-transesterification-activity-of-immobilized-ppd2-lipase/
• ISSN: 0973-7510; 2581-690X

Immobilization of thermostable lipase from Geobacillus thermoleovorans PPD2 (Lip-A) were carried out on Ni-NTA agarose and carboxymethyl chitosan. Free enzyme was obtained by heterologous expression in Escherichia coli as a host cell. The enzyme showed catalytic activity for transesterification reaction. Transesterification activity of immobilized lipase on Ni-NTA agarose was increased by three fold (75.04% conversion) compared to that the free enzyme (24.65%), while the activity of immobilized lipase on carboxymethyl chitosan was slightly decreased (19.86%).