The Effect of Different Matrix Bound on the Transesterification Activity of Immobilized PPD2 Lipase

Immobilization of thermostable lipase from Geobacillus thermoleovorans PPD2 (Lip-A) were carried out on Ni-NTA agarose and carboxymethyl chitosan. Free enzyme was obtained by heterologous expression in Escherichia coli as a host cell. The enzyme showed catalytic activity for transesterification reac...

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Bibliographic Details
Main Authors: Anita Herawati Permana, Fida Madayanti Warganegara, Deana Wahyuningrum, Akhmaloka
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2018-06-01
Series:Journal of Pure and Applied Microbiology
Subjects:
Online Access:https://microbiologyjournal.org/the-effect-of-different-matrix-bound-on-the-transesterification-activity-of-immobilized-ppd2-lipase/