CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair

CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair

The integration of exogenous DNA into the genome using CRISPR–Cas9 often presents a challenge to researchers. Here the authors fuse CtIP to Cas9 to stimulate recombination at target loci.

Bibliographic Details
Main Authors: M. Charpentier, A. H. Y. Khedher, S. Menoret, A. Brion, K. Lamribet, E. Dardillac, C. Boix, L. Perrouault, L. Tesson, S. Geny, A. De Cian, J. M. Itier, I. Anegon, B. Lopez, C. Giovannangeli, J. P. Concordet
Format: Article
Language:English
Published: Nature Publishing Group 2018-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03475-7
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spelling doaj-c444707298f741f68a4178c901271db62021-05-11T10:28:06ZengNature Publishing GroupNature Communications2041-17232018-03-019111110.1038/s41467-018-03475-7CtIP fusion to Cas9 enhances transgene integration by homology-dependent repairM. Charpentier0A. H. Y. Khedher1S. Menoret2A. Brion3K. Lamribet4E. Dardillac5C. Boix6L. Perrouault7L. Tesson8S. Geny9A. De Cian10J. M. Itier11I. Anegon12B. Lopez13C. Giovannangeli14J. P. Concordet15Museum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésMuseum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésCentre de Recherche en Transplantation et Immunologie UMR1064, INSERM, Université de NantesMuseum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésMuseum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésEquipe Labellisée Ligue Contre le Cancer, Institut de Cancérologie Gustave-Roussy, Université Paris-Saclay, CNRS UMR 8200Museum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésMuseum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésCentre de Recherche en Transplantation et Immunologie UMR1064, INSERM, Université de NantesMuseum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésMuseum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésTranslational Sciences, SanofiCentre de Recherche en Transplantation et Immunologie UMR1064, INSERM, Université de NantesEquipe Labellisée Ligue Contre le Cancer, Institut de Cancérologie Gustave-Roussy, Université Paris-Saclay, CNRS UMR 8200Museum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésMuseum National d’Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne UniversitésThe integration of exogenous DNA into the genome using CRISPR–Cas9 often presents a challenge to researchers. Here the authors fuse CtIP to Cas9 to stimulate recombination at target loci.https://doi.org/10.1038/s41467-018-03475-7
collection DOAJ
language English
format Article
sources DOAJ
author M. Charpentier
A. H. Y. Khedher
S. Menoret
A. Brion
K. Lamribet
E. Dardillac
C. Boix
L. Perrouault
L. Tesson
S. Geny
A. De Cian
J. M. Itier
I. Anegon
B. Lopez
C. Giovannangeli
J. P. Concordet
spellingShingle M. Charpentier
A. H. Y. Khedher
S. Menoret
A. Brion
K. Lamribet
E. Dardillac
C. Boix
L. Perrouault
L. Tesson
S. Geny
A. De Cian
J. M. Itier
I. Anegon
B. Lopez
C. Giovannangeli
J. P. Concordet
CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair
Nature Communications
author_facet M. Charpentier
A. H. Y. Khedher
S. Menoret
A. Brion
K. Lamribet
E. Dardillac
C. Boix
L. Perrouault
L. Tesson
S. Geny
A. De Cian
J. M. Itier
I. Anegon
B. Lopez
C. Giovannangeli
J. P. Concordet
author_sort M. Charpentier
title CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair
title_short CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair
title_full CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair
title_fullStr CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair
title_full_unstemmed CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair
title_sort ctip fusion to cas9 enhances transgene integration by homology-dependent repair
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-03-01
description The integration of exogenous DNA into the genome using CRISPR–Cas9 often presents a challenge to researchers. Here the authors fuse CtIP to Cas9 to stimulate recombination at target loci.
url https://doi.org/10.1038/s41467-018-03475-7
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