Generation of recombinant vaccinia virus and analysis of virus-induced cell death

Summary: Vaccinia virus is a large double-stranded DNA virus that is widely used to express foreign genes from different origins. We generated recombinant vaccinia virus that expresses a viral inhibitor to examine its effect on virus-induced necroptosis. We provide a detailed protocol to describe th...

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Bibliographic Details
Main Authors: Zhijun Liu, Kidong Kang, Francis Ka-Ming Chan
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005773