Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp. lactis BGBM50 Strain

• Main Authors: Nemanja Mirkovic, Zorica Radulovic, Gordana Uzelac, Jelena Lozo, Dragojlo Obradovic, Ljubisa Topisirovic, Milan Kojic
• Format: Article
• Language: English
• Published: University of Zagreb 2015-01-01
• Series: Food Technology and Biotechnology
• Subjects: bacteriocin lactococcin G; aggregation-promoting factor; plasmid curing
• Online Access: http://hrcak.srce.hr/file/206936
• ISSN: 1330-9862; 1334-2606

Lactococcus lactis ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Žanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of diff erent sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specifi c for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.