Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method

• Main Authors: Yiping Lin, Yanli Wei, Xiaoxia Hu, Mei-Ling Wu, Jingchan Yao, Xiaoqian Ying, Xiaoyan Fu, Mingxing Ding, Liman Qiao
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2019-03-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: Cocktail; CYP; Herb-drug interaction; Lentinan; Probe drug
• Online Access: http://ijbms.mums.ac.ir/article_12194_e36ea5b976a2f89ce7525a89090adc1e.pdf
• ISSN: 2008-3866; 2008-3874

Objective(s): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. Materials and Methods: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam. Then, we obtained blood in specific durations for the determination of plasma concentration of the probe drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software. Results: We found that various concentrations of lentinan increased the activity of rat CYP1A2, CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9. Conclusion: These findings suggest that clinical application of lentinan combination with CYP3A4, CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug interactions and hence treatment failure.