Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation

Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation

We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (Horlbeck et al., 2016). Here, we build on this observation with a comprehensive algorithm that incorporates chromatin, position, and sequence features to accurately predict highly effective single guide RNAs (sgRNAs) for...

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Bibliographic Details
Main Authors: Max A Horlbeck, Luke A Gilbert, Jacqueline E Villalta, Britt Adamson, Ryan A Pak, Yuwen Chen, Alexander P Fields, Chong Yon Park, Jacob E Corn, Martin Kampmann, Jonathan S Weissman
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2016-09-01
Series:eLife
Subjects:
CRISPR
genetic screening
nucleosomes
Online Access:https://elifesciences.org/articles/19760

Internet

https://elifesciences.org/articles/19760

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