In vitro-transcribed guide RNAs trigger an innate immune response via the RIG-I pathway.

Clustered, regularly interspaced, short palindromic repeat (CRISPR)-CRISPR-associated 9 (Cas9) genome editing is revolutionizing fundamental research and has great potential for the treatment of many diseases. While editing of immortalized cell lines has become relatively easy, editing of therapeuti...

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Bibliographic Details
Main Authors: Beeke Wienert, Jiyung Shin, Elena Zelin, Kathleen Pestal, Jacob E Corn
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-07-01
Series:PLoS Biology
Online Access:http://europepmc.org/articles/PMC6049001?pdf=render