Efficient Cre/loxP Site-Specific Recombination in a HepG2 Human Liver Cell Line
A worldwide shortage of donor livers is a limiting factor of the clinical application of hepatocyte transplantation (HTX). To resolve this issue, we focused on a reversible immortalization system that allows temporary expansion of primary hepatocyte populations by transfer of an oncogene that can be...
Main Authors: | Naoya Kobayashi M.D., Ph.D., Hirofumi Noguchi, Karen A. Westerman, Toshihisa Matsumura, Takamasa Watanabe, Toshinori Totsugawa, Toshiyoshi Fujiwara, Philippe Leboulch, Noriaki Tanaka |
---|---|
Format: | Article |
Language: | English |
Published: |
SAGE Publishing
2000-09-01
|
Series: | Cell Transplantation |
Online Access: | https://doi.org/10.1177/096368970000900525 |
Similar Items
-
Cre/loxP-Based Reversible Immortalization of Human Hepatocytes
by: Naoya Kobayashi M.D., Ph.D., et al.
Published: (2001-05-01) -
Study of covalently closed circular DNA of hepatitis B virus in HepG2 cells by Cre/LoxP system
by: Ju-mei Li, et al.
Published: (2001) -
Fate-mapping the mouse neural tube by Cre-loxP transgenesis
by: Fogarty, Matthew Hugh
Published: (2005) -
Successful Retroviral Gene Transfer of Simian Virus 40 T Antigen and Herpes Simplex Virus-Thymidine Kinase into Human Hepatocytes
by: Naoya Kobayashi M.D., Ph.D., et al.
Published: (2001-05-01) -
Viral Cre-LoxP tools aid genome engineering in mammalian cells
by: Ranjita Sengupta, et al.
Published: (2017-11-01)