Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

Existing examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines wit...

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Main Authors:	Oliver Xiaoou Dong, Shu Yu, Rashmi Jain, Nan Zhang, Phat Q. Duong, Corinne Butler, Yan Li, Anna Lipzen, Joel A. Martin, Kerrie W. Barry, Jeremy Schmutz, Li Tian, Pamela C. Ronald
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-03-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-14981-y

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spelling	doaj-c29b6d170b1a4822ba8073858c6137662021-05-11T08:30:08ZengNature Publishing GroupNature Communications2041-17232020-03-0111111010.1038/s41467-020-14981-yMarker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9Oliver Xiaoou Dong0Shu Yu1Rashmi Jain2Nan Zhang3Phat Q. Duong4Corinne Butler5Yan Li6Anna Lipzen7Joel A. Martin8Kerrie W. Barry9Jeremy Schmutz10Li Tian11Pamela C. Ronald12Department of Plant Pathology and the Genome Center, University of CaliforniaDepartment of Plant Sciences, University of CaliforniaDepartment of Plant Pathology and the Genome Center, University of CaliforniaDepartment of Plant Pathology and the Genome Center, University of CaliforniaDepartment of Plant Pathology and the Genome Center, University of CaliforniaDepartment of Plant Pathology and the Genome Center, University of CaliforniaDepartment of Plant Pathology and the Genome Center, University of CaliforniaDepartment of Energy Joint Genome InstituteDepartment of Energy Joint Genome InstituteDepartment of Energy Joint Genome InstituteDepartment of Energy Joint Genome InstituteDepartment of Plant Sciences, University of CaliforniaDepartment of Plant Pathology and the Genome Center, University of CaliforniaExisting examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines with high carotenoid content.https://doi.org/10.1038/s41467-020-14981-y
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Oliver Xiaoou Dong Shu Yu Rashmi Jain Nan Zhang Phat Q. Duong Corinne Butler Yan Li Anna Lipzen Joel A. Martin Kerrie W. Barry Jeremy Schmutz Li Tian Pamela C. Ronald
spellingShingle	Oliver Xiaoou Dong Shu Yu Rashmi Jain Nan Zhang Phat Q. Duong Corinne Butler Yan Li Anna Lipzen Joel A. Martin Kerrie W. Barry Jeremy Schmutz Li Tian Pamela C. Ronald Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9 Nature Communications
author_facet	Oliver Xiaoou Dong Shu Yu Rashmi Jain Nan Zhang Phat Q. Duong Corinne Butler Yan Li Anna Lipzen Joel A. Martin Kerrie W. Barry Jeremy Schmutz Li Tian Pamela C. Ronald
author_sort	Oliver Xiaoou Dong
title	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_short	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_full	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_fullStr	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_full_unstemmed	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_sort	marker-free carotenoid-enriched rice generated through targeted gene insertion using crispr-cas9
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-03-01
description	Existing examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines with high carotenoid content.
url	https://doi.org/10.1038/s41467-020-14981-y
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Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

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