Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

Existing examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines wit...

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Bibliographic Details
Main Authors: Oliver Xiaoou Dong, Shu Yu, Rashmi Jain, Nan Zhang, Phat Q. Duong, Corinne Butler, Yan Li, Anna Lipzen, Joel A. Martin, Kerrie W. Barry, Jeremy Schmutz, Li Tian, Pamela C. Ronald
Format: Article
Language:English
Published: Nature Publishing Group 2020-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-14981-y

Internet

https://doi.org/10.1038/s41467-020-14981-y

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