Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
Existing examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines wit...
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Bibliographic Details
| Main Authors: |
Oliver Xiaoou Dong,
Shu Yu,
Rashmi Jain,
Nan Zhang,
Phat Q. Duong,
Corinne Butler,
Yan Li,
Anna Lipzen,
Joel A. Martin,
Kerrie W. Barry,
Jeremy Schmutz,
Li Tian,
Pamela C. Ronald |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Publishing Group
2020-03-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-020-14981-y
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