Prevalence of tst, entC, entA and entA/C genes in staphylococcus aureus strains isolated from different foods

• Main Authors: Soltan Dallal MM, Seyed Amiri S, Mardani N, Abedi Mohtasab TP, Rahimi Forushani A, Pourmand MR, Salehipour Z, Eshraghi S, Bakhtyari R, Agha Amiri S, Zahraei Salehi MT
• Format: Article
• Language: fas
• Published: Tehran University of Medical Sciences 2009-10-01
• Series: Tehran University Medical Journal
• Subjects: Staphylococcus aureus; toxic shock syndrome toxin-1; enterotoxin A; enterotoxin C
• Online Access: http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14587.pdf&manuscript_id=14587

"nBackground: Staphylococcus aureus is a major foodborne pathogen throughout the world. Enterotoxins and toxic shock syndrome toxin-1 are important virulence factors and as pyrogenic toxin superantigens have profound effects on the ir host. Thus circulation of TSST1 producing S.aureus among people and food chain is a worrying issue. The present paper was conducted to study Prevalence of tst, entC, entA and entA/C genes in staphylococcus aureus strains isolated from different foods. "n"nMethods: Over 1040 food samples have been analyzed differentially according to Iran national standard (number= 1194) for S.aureus identification. After DNA extraction, PCR reactions were carried out by reference strain as positive control, adequate primers. "n"nResults: At present study, prevalence of foodstuffs contaminated by S.aureus isolates was about 9.5% (100 strains). Of 25% of isolates producing entC, 28% (seven strains) had tst gene at the same time and of 8% of isolates producing entA, 12.5% (one strain) were positive for tst genes simultaneously. Altogether of 9% isolates producing combination of entC and entA, 44.4% (four strains) were also producer of tst gene. "n"nConclusion: Prevalence of TSST1 producing strains in combination with enterotoxin genes is considerable especially with entC and A plus C. On the other hand, circulation of these isolates in humans, animals, foods and environment has hazardous effect for general public health.