A non-fluorescent HaloTag blocker for improved measurement and visualization of protein synthesis in living cells [version 2; peer review: 2 approved]
Background: HaloTag is a modified bacterial enzyme that binds rapidly and irreversibly to an array of synthetic ligands, including chemical dyes. When expressed in live cells in conjunction with a protein of interest, HaloTag can be used to study protein trafficking, synthesis, and degradation. For...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
F1000 Research Ltd
2020-06-01
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| Series: | F1000Research |
| Online Access: | https://f1000research.com/articles/9-302/v2 |