The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps

The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps

ABSTRACT Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K ha...

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Bibliographic Details
Main Authors: ARTURO B. MARTINELLI, CECILIA WAICHERT, DIEGO N. BARBOSA, VALÉRIA FAGUNDES, CELSO O. AZEVEDO
Format: Article
Language:English
Published: Academia Brasileira de Ciências 2017-08-01
Series:Anais da Academia Brasileira de Ciências
Subjects:
Diaphanization
genitalia preparation
KOH
Wolbachia
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005020109&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005020109&lng=en&tlng=en

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