Single-step purification of crotapotin and crotactine from Crotalus durissus terrificus venom using preparative isoelectric focusing
We describe the isolation of crotoxin, a presynaptic B-neurotoxin, as well as its subunits B (crotactine) and A (crotapotin) from lyophilized Crotalus durissus terrificus venom by a single-step preparative isoelectric focusing procedure. From 98 mg of dried venom protein 20.1 mg of crotactine and 13...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
1997-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100004 |