Systematic design of 18S rRNA gene primers for determining eukaryotic diversity in microbial consortia.

High-throughput sequencing of ribosomal RNA gene (rDNA) amplicons has opened up the door to large-scale comparative studies of microbial community structures. The short reads currently produced by massively parallel sequencing technologies make the choice of sequencing region crucial for accurate ph...

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Bibliographic Details
Main Authors: Luisa W Hugerth, Emilie E L Muller, Yue O O Hu, Laura A M Lebrun, Hugo Roume, Daniel Lundin, Paul Wilmes, Anders F Andersson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24755918/?tool=EBI