Rapid Detection and Quantification of Mycobacterium tuberculosis DNA in Paraffinized Samples by Droplet Digital PCR: A Preliminary Study

Rapid Detection and Quantification of Mycobacterium tuberculosis DNA in Paraffinized Samples by Droplet Digital PCR: A Preliminary Study

Background: Rapid and reliable diagnosis of tuberculosis (TB) represents a diagnostic challenge in compartmentalized extrapulmonary TB infection because of the small number of mycobacteria (MTB) and the frequent lack of fresh samples to perform culture. Here, we estimate the performances of homemade...

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Bibliographic Details
Main Authors: Maria Antonello, Rossana Scutari, Calogero Lauricella, Silvia Renica, Valentina Motta, Stefania Torri, Cristina Russo, Leonarda Gentile, Valeria Cento, Luna Colagrossi, Giordana Mattana, Luigi Ruffo Codecasa, Chiara Vismara, Francesco Scaglione, Silvio Marco Veronese, Emanuela Bonoldi, Alessandra Bandera, Andrea Gori, Ester Mazzola, Carlo Federico Perno, Claudia Alteri
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Microbiology
Subjects:
MTB
ddPCR
MTB diagnosis
tuberculosis
extrapulmonary TB
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.727774/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.727774/full

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