Optimization and Validation of a Sensitive HPLC–PDA Method for Simultaneous Determination of Torasemide and Spironolactone in Human Plasma using Central Composite Design

Optimization and Validation of a Sensitive HPLC–PDA Method for Simultaneous Determination of Torasemide and Spironolactone in Human Plasma using Central Composite Design

A sensitive, accurate, precise and rapid HPLC-PDA method was developed and validated for the simultaneous determination of torasemide and spironolactone in human plasma using Design of experiments. Central composite design was used to optimize the method using content of acetonitrile, concentration...

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Bibliographic Details
Main Authors: Venkatesan Subramanian, Kannappan Nagappan, Sai Sandeep Mannemala
Format: Article
Language:English
Published: Slovenian Chemical Society 2015-03-01
Series:Acta Chimica Slovenica
Subjects:
Column liquid Chromatography
Torasemide
Spironolactone
Human plasma
Bioanalytical method validation
Central Composite Design
Online Access:https://journals.matheo.si/index.php/ACSi/article/view/1262

Internet

https://journals.matheo.si/index.php/ACSi/article/view/1262

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