Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences

Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences

Abstract Background Determining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate representation is dependent on unbiased PCR amplification post bisulfite modification, regardless of methylation status o...

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Bibliographic Details
Main Authors: Ida L. M. Candiloro, Thomas Mikeska, Alexander Dobrovic
Format: Article
Language:English
Published: BMC 2017-04-01
Series:Clinical Epigenetics
Subjects:
Increase Annealing Temperature
Lower Annealing Temperature
Abasic Site
Bisulfite Modification
Bisulfite Pyrosequencing
Online Access:http://link.springer.com/article/10.1186/s13148-017-0328-4

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http://link.springer.com/article/10.1186/s13148-017-0328-4

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