Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit

Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit

Abstract Background Plasmid-borne genetic editing tools, including the widely used CRISPR-Cas9 system, have greatly facilitated bacterial programming to obtain novel functionalities. However, the lack of effective post-editing plasmid elimination methods impedes follow-up genetic manipulation or app...

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Bibliographic Details
Main Authors: Qiang Tang, Chunbo Lou, Shuang-Jiang Liu
Format: Article
Language:English
Published: BMC 2017-09-01
Series:Journal of Biological Engineering
Subjects:
EXIT circuit
I-SceI
Plasmid elimination
One-step
CRISPR-Cas9
Easy-to-use
Online Access:http://link.springer.com/article/10.1186/s13036-017-0072-5

Internet

http://link.springer.com/article/10.1186/s13036-017-0072-5

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