Guide RNAs with embedded barcodes boost CRISPR-pooled screens

Guide RNAs with embedded barcodes boost CRISPR-pooled screens

Abstract We report a new method using re-designed guide RNAs with internal barcodes (iBARs) embedded in their loop regions. Our iBAR approach outperforms the conventional method by producing screening results with much lower false-positive and false-negative rates especially with a high multiplicity...

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Bibliographic Details
Main Authors: Shiyou Zhu, Zhongzheng Cao, Zhiheng Liu, Yuan He, Yinan Wang, Pengfei Yuan, Wei Li, Feng Tian, Ying Bao, Wensheng Wei
Format: Article
Language:English
Published: BMC 2019-01-01
Series:Genome Biology
Online Access:http://link.springer.com/article/10.1186/s13059-019-1628-0

Internet

http://link.springer.com/article/10.1186/s13059-019-1628-0

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