Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress
Heat shock proteins (HSPs), originally identified as heat-inducible gene products, are a family of highly conserved proteins that respond to a wide variety of stress including oxidative stress. Although both acute and chronic oxidative stress have been well demonstrated to induce HSP responses, lit...
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2014-01-01
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| Series: | Redox Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231714001098 |
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doaj-bd73ff5494334ac4a6297722fc5c6cae2020-11-25T02:03:37ZengElsevierRedox Biology2213-23172014-01-013C252810.1016/j.redox.2014.10.003Natural thermal adaptation increases heat shock protein levels and decreases oxidative stressNiku K.J. Oksala0F. Güler Ekmekçi1Ergi Özsoy2Şerife Kirankaya3Tarja Kokkola4Güzin Emecen5Jani Lappalainen6Kai Kaarniranta7Mustafa Atalay8Institute of Biomedicine, Department of Physiology, University of Eastern Finland, Kuopio, FinlandDepartment of Biology, Faculty of Science, University of Hacettepe, Beytepe, TurkeyDepartment of Biology, Faculty of Science, University of Hacettepe, Beytepe, TurkeyDepartment of Biology, Faculty of Science and Literature, University of Düzce, Düzce, TurkeyInstitute of Biomedicine, Department of Physiology, University of Eastern Finland, Kuopio, FinlandDepartment of Biology, Faculty of Science, University of Hacettepe, Beytepe, TurkeyInstitute of Biomedicine, Department of Physiology, University of Eastern Finland, Kuopio, FinlandDepartment of Ophthalmology, Institute of Clinical Medicine, University of Eastern Finland, FinlandInstitute of Biomedicine, Department of Physiology, University of Eastern Finland, Kuopio, Finland Heat shock proteins (HSPs), originally identified as heat-inducible gene products, are a family of highly conserved proteins that respond to a wide variety of stress including oxidative stress. Although both acute and chronic oxidative stress have been well demonstrated to induce HSP responses, little evidence is available whether increased HSP levels provide enhanced protection against oxidative stress under elevated yet sublethal temperatures. We studied relationships between oxidative stress and HSPs in a physiological model by using Garra rufa (doctor fish), a fish species naturally acclimatized to different thermal conditions. We compared fish naturally living in a hot spring with relatively high water temperature (34.4±0.6 °C) to those living in normal river water temperature (25.4±4.7 °C), and found that levels of all the studied HSPs (HSP70, HSP60, HSP90, HSC70 and GRP75) were higher in fish living in elevated water temperature compared with normal river water temperature. In contrast, indicators of oxidative stress, including protein carbonyls and lipid hydroperoxides, were decreased in fish living in the elevated temperature, indicating that HSP levels are inversely associated with oxidative stress. The present results provide evidence that physiologically increased HSP levels provide protection against oxidative stress and enhance cytoprotection. http://www.sciencedirect.com/science/article/pii/S2213231714001098ThermalOxidationStressRegulationGarra rufa |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Niku K.J. Oksala F. Güler Ekmekçi Ergi Özsoy Şerife Kirankaya Tarja Kokkola Güzin Emecen Jani Lappalainen Kai Kaarniranta Mustafa Atalay |
| spellingShingle |
Niku K.J. Oksala F. Güler Ekmekçi Ergi Özsoy Şerife Kirankaya Tarja Kokkola Güzin Emecen Jani Lappalainen Kai Kaarniranta Mustafa Atalay Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress Redox Biology Thermal Oxidation Stress Regulation Garra rufa |
| author_facet |
Niku K.J. Oksala F. Güler Ekmekçi Ergi Özsoy Şerife Kirankaya Tarja Kokkola Güzin Emecen Jani Lappalainen Kai Kaarniranta Mustafa Atalay |
| author_sort |
Niku K.J. Oksala |
| title |
Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress |
| title_short |
Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress |
| title_full |
Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress |
| title_fullStr |
Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress |
| title_full_unstemmed |
Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress |
| title_sort |
natural thermal adaptation increases heat shock protein levels and decreases oxidative stress |
| publisher |
Elsevier |
| series |
Redox Biology |
| issn |
2213-2317 |
| publishDate |
2014-01-01 |
| description |
Heat shock proteins (HSPs), originally identified as heat-inducible gene products, are a family of highly conserved proteins that respond to a wide variety of stress including oxidative stress. Although both acute and chronic oxidative stress have been well demonstrated to induce HSP responses, little evidence is available whether increased HSP levels provide enhanced protection against oxidative stress under elevated yet sublethal temperatures. We studied relationships between oxidative stress and HSPs in a physiological model by using Garra rufa (doctor fish), a fish species naturally acclimatized to different thermal conditions. We compared fish naturally living in a hot spring with relatively high water temperature (34.4±0.6 °C) to those living in normal river water temperature (25.4±4.7 °C), and found that levels of all the studied HSPs (HSP70, HSP60, HSP90, HSC70 and GRP75) were higher in fish living in elevated water temperature compared with normal river water temperature. In contrast, indicators of oxidative stress, including protein carbonyls and lipid hydroperoxides, were decreased in fish living in the elevated temperature, indicating that HSP levels are inversely associated with oxidative stress. The present results provide evidence that physiologically increased HSP levels provide protection against oxidative stress and enhance cytoprotection.
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| topic |
Thermal Oxidation Stress Regulation Garra rufa |
| url |
http://www.sciencedirect.com/science/article/pii/S2213231714001098 |
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