A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)

A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)

Abstract Background Genome editing of monocot plants can be accomplished by using the components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9) technology specifically optimized for these types of plants. Here, we present the development of RNA-g...

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Bibliographic Details
Main Authors: Sebastian Gasparis, Maciej Kała, Mateusz Przyborowski, Leszek A. Łyżnik, Wacław Orczyk, Anna Nadolska-Orczyk
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Plant Methods
Subjects:
CRISPR/Cas9
Genome editing
Barley
PTG
CKX genes
Online Access:http://link.springer.com/article/10.1186/s13007-018-0382-8

Internet

http://link.springer.com/article/10.1186/s13007-018-0382-8

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