Stepwise partially overlapping primer-based PCR for genome walking

Stepwise partially overlapping primer-based PCR for genome walking

Abstract A stepwise partially overlapping primer-based PCR (SWPOP-PCR) method for isolating flanking unknown DNA regions was developed, which comprises three rounds of nested PCRs sequentially driven by SWPOP primer-nested specific primer pairs. SWPOP primer set is characterized by a partial overlap...

Full description

Bibliographic Details
Main Authors: Kunpeng Chang, Qiong Wang, Xiaofei Shi, Shuixing Wang, Hongjing Wu, Lijuan Nie, Haixing Li
Format: Article
Language:English
Published: SpringerOpen 2018-05-01
Series:AMB Express
Subjects:
Partially overlapping primer
PCR
Genome walking
gadA locus
hyg
Online Access:http://link.springer.com/article/10.1186/s13568-018-0610-7

Internet

http://link.springer.com/article/10.1186/s13568-018-0610-7

Similar Items