Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.

The basic research in cell biology and in medical sciences makes large use of imaging tools mainly based on confocal fluorescence and, more recently, on non-linear excitation microscopy. Substantially the aim is the recognition of selected targets in the image and their tracking in time. We have dev...

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Bibliographic Details
Main Authors: Carlo E Villa, Michele Caccia, Laura Sironi, Laura D'Alfonso, Maddalena Collini, Ilaria Rivolta, Giuseppe Miserocchi, Tatiana Gorletta, Ivan Zanoni, Francesca Granucci, Giuseppe Chirico
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-08-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808918/?tool=EBI