An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

Summary: The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provid...

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Bibliographic Details
Main Authors: Davi Marco Lyra-Leite, Hananeh Fonoudi, Mennat Gharib, Paul W. Burridge
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302008