Allele-Specific PCR for <i>KRAS</i> Mutation Detection Using Phosphoryl Guanidine Modified Primers

Allele-Specific PCR for <i>KRAS</i> Mutation Detection Using Phosphoryl Guanidine Modified Primers

Establishing the Kirsten rat sarcoma <i>(KRAS</i>) mutational status is essential in terms of managing patients with various types of cancer. Allele-specific real-time polymerase chain reaction (AS-PCR) is a widely used method for somatic mutations detection. To improve the limited sensi...

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Bibliographic Details
Main Authors: Alexey S. Chubarov, Igor P. Oscorbin, Maxim L. Filipenko, Alexander A. Lomzov, Dmitrii V. Pyshnyi
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Diagnostics
Subjects:
mutation detection
<i>KRAS</i> mutations
allele-specific PCR
blocker PCR
modified oligonucleotides
phosphoryl guanidine oligonucleotide (PGO)
Online Access:https://www.mdpi.com/2075-4418/10/11/872

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https://www.mdpi.com/2075-4418/10/11/872

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