Residue proximity information and protein model discrimination using saturation-suppressor mutagenesis

Residue proximity information and protein model discrimination using saturation-suppressor mutagenesis

Identification of residue-residue contacts from primary sequence can be used to guide protein structure prediction. Using Escherichia coli CcdB as the test case, we describe an experimental method termed saturation-suppressor mutagenesis to acquire residue contact information. In this methodology, f...

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Bibliographic Details
Main Authors: Anusmita Sahoo, Shruti Khare, Sivasankar Devanarayanan, Pankaj C. Jain, Raghavan Varadarajan
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2015-12-01
Series:eLife
Subjects:
protein stability
Pairwise contacts
model discrimination
structure prediction
Online Access:https://elifesciences.org/articles/09532

Internet

https://elifesciences.org/articles/09532

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