Highly sensitive quantification of serum malonate, a possible marker for de novo lipogenesis, by LC-ESI-MS/MS

Highly sensitive quantification of serum malonate, a possible marker for de novo lipogenesis, by LC-ESI-MS/MS

We describe a new sensitive and specific method for the quantification of serum malonate (malonic acid, MA), which could be a new biomarker for de novo lipogenesis (fatty acid synthesis). This method is based upon a stable isotope-dilution technique using LC-MS/MS. MA from 50 μl of serum was derivat...

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Bibliographic Details
Main Authors: Akira Honda, Kouwa Yamashita, Tadashi Ikegami, Takashi Hara, Teruo Miyazaki, Takeshi Hirayama, Mitsuteru Numazawa, Yasushi Matsuzaki
Format: Article
Language:English
Published: Elsevier 2009-10-01
Series:Journal of Lipid Research
Subjects:
acetyl-CoA carboxylase
carnitine palmitoyl transferase 1
fatty acid synthase
liquid chromatography-electrospray ionization-tandem mass spectrometry
malonic acid
malonyl-CoA
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520307215

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http://www.sciencedirect.com/science/article/pii/S0022227520307215

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