Convenience versus Biological Significance: Are PMA-Differentiated THP-1 Cells a Reliable Substitute for Blood-Derived Macrophages When Studying in Vitro Polarization?

Convenience versus Biological Significance: Are PMA-Differentiated THP-1 Cells a Reliable Substitute for Blood-Derived Macrophages When Studying in Vitro Polarization?

Human peripheral-blood monocytes are used as an established in vitro system for generating macrophages. For several reasons, monocytic cell lines such as THP-1 have been considered as a possible alternative. In view of their distinct developmental origins and phenotypic attributes, we set out to ass...

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Bibliographic Details
Main Authors: Serena Tedesco, Federica De Majo, Jieun Kim, Annalisa Trenti, Lucia Trevisi, Gian Paolo Fadini, Chiara Bolego, Peter W. Zandstra, Andrea Cignarella, Libero Vitiello
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Pharmacology
Subjects:
macrophage activation
phagocytosis
phenotype
THP-1 macrophages
human macrophages
migration
Online Access:http://journal.frontiersin.org/article/10.3389/fphar.2018.00071/full

Internet

http://journal.frontiersin.org/article/10.3389/fphar.2018.00071/full

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