Improved sensitivity of an acid sphingomyelinase activity assay using a C6:0 sphingomyelin substrate

Improved sensitivity of an acid sphingomyelinase activity assay using a C6:0 sphingomyelin substrate

Short-chain C6-sphingomyelin is an artificial substrate that was used in an acid sphingomyelinase activity assay for a pilot screening study of patients with Niemann–Pick disease types A and B. Using previously published multiplex and single assay conditions, normal acid sphingomyelinase activity le...

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Bibliographic Details
Main Authors: Wei-Lien Chuang, Joshua Pacheco, Samantha Cooper, Jonathan S. Kingsbury, John Hinds, Pavlina Wolf, Petra Oliva, Joan Keutzer, Gerald F. Cox, Kate Zhang
Format: Article
Language:English
Published: Elsevier 2015-06-01
Series:Molecular Genetics and Metabolism Reports
Subjects:
Niemann–Pick disease type A/B
Acid sphingomyelinase activity
Screening
Natural substrate
Online Access:http://www.sciencedirect.com/science/article/pii/S2214426915300057

Internet

http://www.sciencedirect.com/science/article/pii/S2214426915300057

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