The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval

Recent evidence suggests repeated mild brain trauma may result in cumulative damage. We investigated cell damage and death in hippocampal cultures following repeated mechanical trauma in vitro by measuring propidium iodide (PrI) uptake, release of neuron-specific enolase (NSE) and glial S-100β prote...

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Main Authors:	Jennifer E. Slemmer, John T. Weber
Format:	Article
Language:	English
Published:	Elsevier 2005-04-01
Series:	Neurobiology of Disease
Subjects:	Glia In vitro injury NSE Repetitive injury S-100β Traumatic brain injury
Online Access:	http://www.sciencedirect.com/science/article/pii/S0969996104002335

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spelling	doaj-ba3729a2f11242289c8378a7df44f8d22021-03-20T04:50:23ZengElsevierNeurobiology of Disease1095-953X2005-04-01183421431The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury intervalJennifer E. Slemmer0John T. Weber1Department of Neuroscience, Erasmus Medical Center, PO Box 1738, 3000 DR Rotterdam, The NetherlandsCorresponding author. Fax: +31 10 408 9459.; Department of Neuroscience, Erasmus Medical Center, PO Box 1738, 3000 DR Rotterdam, The NetherlandsRecent evidence suggests repeated mild brain trauma may result in cumulative damage. We investigated cell damage and death in hippocampal cultures following repeated mechanical trauma in vitro by measuring propidium iodide (PrI) uptake, release of neuron-specific enolase (NSE) and glial S-100β protein, and performing neuronal counts. Cultures receiving two mild injuries (31% stretch) 1 or 24 h apart displayed different profiles of PrI uptake and S-100β release, although neuronal loss and NSE release was similar in both paradigms. Cells receiving a subthreshold, low-level stretch (10%) repeated several times eventually stained with PrI. Cultures administered 10% stretch before mild injury released less S-100β than mild injury alone, suggesting a preconditioning effect. Lastly, exogenous S-100β applied to injured cultures decreased PrI uptake, implying a protective role. These results suggest cumulative damage is dependent on injury severity and inter-injury interval, and that neurons and glia react differently to various injury paradigms.http://www.sciencedirect.com/science/article/pii/S0969996104002335GliaIn vitro injuryNSERepetitive injuryS-100βTraumatic brain injury
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jennifer E. Slemmer John T. Weber
spellingShingle	Jennifer E. Slemmer John T. Weber The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval Neurobiology of Disease Glia In vitro injury NSE Repetitive injury S-100β Traumatic brain injury
author_facet	Jennifer E. Slemmer John T. Weber
author_sort	Jennifer E. Slemmer
title	The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval
title_short	The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval
title_full	The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval
title_fullStr	The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval
title_full_unstemmed	The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval
title_sort	extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval
publisher	Elsevier
series	Neurobiology of Disease
issn	1095-953X
publishDate	2005-04-01
description	Recent evidence suggests repeated mild brain trauma may result in cumulative damage. We investigated cell damage and death in hippocampal cultures following repeated mechanical trauma in vitro by measuring propidium iodide (PrI) uptake, release of neuron-specific enolase (NSE) and glial S-100β protein, and performing neuronal counts. Cultures receiving two mild injuries (31% stretch) 1 or 24 h apart displayed different profiles of PrI uptake and S-100β release, although neuronal loss and NSE release was similar in both paradigms. Cells receiving a subthreshold, low-level stretch (10%) repeated several times eventually stained with PrI. Cultures administered 10% stretch before mild injury released less S-100β than mild injury alone, suggesting a preconditioning effect. Lastly, exogenous S-100β applied to injured cultures decreased PrI uptake, implying a protective role. These results suggest cumulative damage is dependent on injury severity and inter-injury interval, and that neurons and glia react differently to various injury paradigms.
topic	Glia In vitro injury NSE Repetitive injury S-100β Traumatic brain injury
url	http://www.sciencedirect.com/science/article/pii/S0969996104002335
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The extent of damage following repeated injury to cultured hippocampal cells is dependent on the severity of insult and inter-injury interval

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