Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives

Enzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during...

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Main Authors: Erica Cruz, Joao Batista Barbosa, Simone Vilela Talma, Edite Andrade Costa, Meire Lelis Leal Martins
Format: Article
Language:Portuguese
Published: Associação Acadêmica de Propriedade Intelectual 2019-03-01
Series:Revista GEINTEC
Online Access:http://www.revistageintec.net/index.php/revista/article/view/1377
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spelling doaj-b6b0b17428e7420d9fbb3def1d58f58d2020-11-24T21:51:59ZporAssociação Acadêmica de Propriedade IntelectualRevista GEINTEC2237-07222019-03-01914740474410.7198/geintec.v9i1.1377708Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additivesErica Cruz0Joao Batista BarbosaSimone Vilela TalmaEdite Andrade CostaMeire Lelis Leal MartinsUniversidade Luterana do BrasilEnzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during storage for 154 days at 3 °C. The activity of avicellase and CMCase was maintained when crude extracts containing the enzymes were incubated in the presence of ethylene glycol (1M), glycerol (1%) and polyethylene glycol (0.01M) for 1 hour at room temperature.http://www.revistageintec.net/index.php/revista/article/view/1377
collection DOAJ
language Portuguese
format Article
sources DOAJ
author Erica Cruz
Joao Batista Barbosa
Simone Vilela Talma
Edite Andrade Costa
Meire Lelis Leal Martins
spellingShingle Erica Cruz
Joao Batista Barbosa
Simone Vilela Talma
Edite Andrade Costa
Meire Lelis Leal Martins
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives
Revista GEINTEC
author_facet Erica Cruz
Joao Batista Barbosa
Simone Vilela Talma
Edite Andrade Costa
Meire Lelis Leal Martins
author_sort Erica Cruz
title Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives
title_short Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives
title_full Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives
title_fullStr Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives
title_full_unstemmed Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives
title_sort stability cellulases of bacillus sp. smia-2 during storage under freezing and in the presence of additives
publisher Associação Acadêmica de Propriedade Intelectual
series Revista GEINTEC
issn 2237-0722
publishDate 2019-03-01
description Enzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during storage for 154 days at 3 °C. The activity of avicellase and CMCase was maintained when crude extracts containing the enzymes were incubated in the presence of ethylene glycol (1M), glycerol (1%) and polyethylene glycol (0.01M) for 1 hour at room temperature.
url http://www.revistageintec.net/index.php/revista/article/view/1377
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AT editeandradecosta stabilitycellulasesofbacillusspsmia2duringstorageunderfreezingandinthepresenceofadditives
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