Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives

Enzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during...

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Bibliographic Details
Main Authors: Erica Cruz, Joao Batista Barbosa, Simone Vilela Talma, Edite Andrade Costa, Meire Lelis Leal Martins
Format: Article
Language:Portuguese
Published: Associação Acadêmica de Propriedade Intelectual 2019-03-01
Series:Revista GEINTEC
Online Access:http://www.revistageintec.net/index.php/revista/article/view/1377
Description
Summary:Enzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during storage for 154 days at 3 °C. The activity of avicellase and CMCase was maintained when crude extracts containing the enzymes were incubated in the presence of ethylene glycol (1M), glycerol (1%) and polyethylene glycol (0.01M) for 1 hour at room temperature.
ISSN:2237-0722