Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin
Nebulin is about 800 kDa filamentous protein that binds the entire thin filament of vertebrate skeletal muscle sarcomeres. Nebulin cannot be isolated from muscle except in a completely denatured form by direct solubilization of myofibrils with SDS because nebulin is hardly soluble under salt conditi...
Main Authors: | Ryo Chitose, Atsushi Watanabe, Masato Asano, Akira Hanashima, Kouhei Sasano, Yulong Bao, Koscak Maruyama, Sumiko Kimura |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2010-01-01
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Series: | Journal of Biomedicine and Biotechnology |
Online Access: | http://dx.doi.org/10.1155/2010/108495 |
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