Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin

Nebulin is about 800 kDa filamentous protein that binds the entire thin filament of vertebrate skeletal muscle sarcomeres. Nebulin cannot be isolated from muscle except in a completely denatured form by direct solubilization of myofibrils with SDS because nebulin is hardly soluble under salt conditi...

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Bibliographic Details
Main Authors: Ryo Chitose, Atsushi Watanabe, Masato Asano, Akira Hanashima, Kouhei Sasano, Yulong Bao, Koscak Maruyama, Sumiko Kimura
Format: Article
Language:English
Published: Hindawi Limited 2010-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2010/108495