Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

• Main Authors: Demetra S.M. Chatzileontiadou, Christopher Szeto, Dhilshan Jayasinghe, Stephanie Gras
• Format: Article
• Language: English
• Published: Elsevier 2021-09-01
• Series: STAR Protocols
• Subjects: Immunology; Protein expression and purification; X-ray Crystallography
• Online Access: http://www.sciencedirect.com/science/article/pii/S2666166721003427
• ISSN: 2666-1667

Summary: Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides.For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).