Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

Summary: Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequ...

Full description

Bibliographic Details
Main Authors: Demetra S.M. Chatzileontiadou, Christopher Szeto, Dhilshan Jayasinghe, Stephanie Gras
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Immunology
Protein expression and purification
X-ray Crystallography
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003427

Internet

http://www.sciencedirect.com/science/article/pii/S2666166721003427

Similar Items