Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component

Ascaris suum allergenic components (PIII) separated by gel filtration chromatography of an adult worm extract were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells using polyethylene glycol (MW 1450) as fusogen. The hybrido...

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Bibliographic Details
Main Authors: R.R. Pires, T.M. Oshiro, D.M. Itami, I. Fernandes, M.F. Macedo-Soares
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2001-08-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
IgE
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800009