Investigation on the optimal wavelength for two-photon microscopy in brain tissue
Two-photon microscopy (TPM) has been one of the most widely used fluorescent microscopy methods in biological tissue. The femtosecond laser in the near-infrared (NIR) range showed better penetration in tissue due to less scattering and absorption. The method to estimate the optimal wavelength genera...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
AIP Publishing LLC
2018-06-01
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Series: | AIP Advances |
Online Access: | http://dx.doi.org/10.1063/1.5025808 |