Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2016-08-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms12463 |