Selective immunocapture and light-controlled traceless release of transiently caged proteins

Selective immunocapture and light-controlled traceless release of transiently caged proteins

Summary: The 4,5-dimethoxy-2-nitrobenzyl (DMNB) photocaging group introduced into small biomolecules, peptides, oligonucleotides, and proteins is commonly used for spatiotemporal control of chemical and biological processes. Here, we describe the use of a DMNB-selective monoclonal antibody for non-c...

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Main Authors: Rasa Rakauskaitė, Giedrė Urbanavičiūtė, Martynas Simanavičius, Aurelija Žvirblienė, Saulius Klimašauskas
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Antibody
Molecular/Chemical Probes
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001623
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spelling doaj-b4ce92ef8f5d4fe49586b9bbea7951ca2021-06-21T04:25:13ZengElsevierSTAR Protocols2666-16672021-06-0122100455Selective immunocapture and light-controlled traceless release of transiently caged proteinsRasa Rakauskaitė0Giedrė Urbanavičiūtė1Martynas Simanavičius2Aurelija Žvirblienė3Saulius Klimašauskas4Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT-10257, Lithuania; Corresponding authorInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT-10257, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT-10257, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT-10257, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT-10257, Lithuania; Corresponding authorSummary: The 4,5-dimethoxy-2-nitrobenzyl (DMNB) photocaging group introduced into small biomolecules, peptides, oligonucleotides, and proteins is commonly used for spatiotemporal control of chemical and biological processes. Here, we describe the use of a DMNB-selective monoclonal antibody for non-covalent capture of chemically or biosynthetically produced proteins containing surface-exposed DMNB caging groups followed by light-controlled traceless decaging and release of the bound proteins into solution for a variety of downstream applications.For complete details on the use and execution of this protocol, please refer to Rakauskaitė et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721001623AntibodyMolecular/Chemical ProbesProtein Biochemistry
collection DOAJ
language English
format Article
sources DOAJ
author Rasa Rakauskaitė
Giedrė Urbanavičiūtė
Martynas Simanavičius
Aurelija Žvirblienė
Saulius Klimašauskas
spellingShingle Rasa Rakauskaitė
Giedrė Urbanavičiūtė
Martynas Simanavičius
Aurelija Žvirblienė
Saulius Klimašauskas
Selective immunocapture and light-controlled traceless release of transiently caged proteins
STAR Protocols
Antibody
Molecular/Chemical Probes
Protein Biochemistry
author_facet Rasa Rakauskaitė
Giedrė Urbanavičiūtė
Martynas Simanavičius
Aurelija Žvirblienė
Saulius Klimašauskas
author_sort Rasa Rakauskaitė
title Selective immunocapture and light-controlled traceless release of transiently caged proteins
title_short Selective immunocapture and light-controlled traceless release of transiently caged proteins
title_full Selective immunocapture and light-controlled traceless release of transiently caged proteins
title_fullStr Selective immunocapture and light-controlled traceless release of transiently caged proteins
title_full_unstemmed Selective immunocapture and light-controlled traceless release of transiently caged proteins
title_sort selective immunocapture and light-controlled traceless release of transiently caged proteins
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-06-01
description Summary: The 4,5-dimethoxy-2-nitrobenzyl (DMNB) photocaging group introduced into small biomolecules, peptides, oligonucleotides, and proteins is commonly used for spatiotemporal control of chemical and biological processes. Here, we describe the use of a DMNB-selective monoclonal antibody for non-covalent capture of chemically or biosynthetically produced proteins containing surface-exposed DMNB caging groups followed by light-controlled traceless decaging and release of the bound proteins into solution for a variety of downstream applications.For complete details on the use and execution of this protocol, please refer to Rakauskaitė et al. (2020).
topic Antibody
Molecular/Chemical Probes
Protein Biochemistry
url http://www.sciencedirect.com/science/article/pii/S2666166721001623
work_keys_str_mv AT rasarakauskaite selectiveimmunocaptureandlightcontrolledtracelessreleaseoftransientlycagedproteins
AT giedreurbanaviciute selectiveimmunocaptureandlightcontrolledtracelessreleaseoftransientlycagedproteins
AT martynassimanavicius selectiveimmunocaptureandlightcontrolledtracelessreleaseoftransientlycagedproteins
AT aurelijazvirbliene selectiveimmunocaptureandlightcontrolledtracelessreleaseoftransientlycagedproteins
AT sauliusklimasauskas selectiveimmunocaptureandlightcontrolledtracelessreleaseoftransientlycagedproteins
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